In-booth presentations
We will present a further step in cryo-electron microscope technology development. Analogous to the development of the automated Thermo Scientific Krios Cryo-TEM approximately 15 years ago, we will discuss a next-generation cryo-FIB system. The new instrument is dedicated to production of cryo-lamellae for the cryo-ET workflow. It features a state-of-the-art plasma ion source, an automated sample loading system, integrated fluorescence microscopy, and the lowest contamination rates, allowing preparation of cryo-lamellae at a higher level of productivity than ever before, thus producing the best possible samples for tomography.
We will show some of the highest resolution structures of proteins obtained either from purified samples or directly from cellular lamellas using a Thermo Scientific Krios G4 Cryo-TEM equipped with the Selectris X Imaging Filter and Falcon 4i Direct Electron Detector. In addition, we will show a high-throughput, semi-automated workflow that enables preparation of cellular lamella and data collection with Thermo Scientific TEM Tomography 5 Software, as well as our new on-the-fly tilt series alignment and reconstruction pipeline with Thermo Scientific Tomo Live Software.
Understanding both the chemistry and structure of surfaces can be key to understanding how they behave in an application. Join us for this presentation to see how data from XPS instruments, such as the Thermo Scientific Nexsa G2 Surface Analysis System, can be combined with imaging from SEMs by using Thermo Scientific Maps Software. We'll show just how easy it is to correlate the data from the two instruments using our dedicated Thermo Scientific CISA Workflow.
Meet our new cryo-transmission electron microscope, the Thermo Scientific Tundra Cryo-TEM, dedicated to single particle analysis (SPA). It features state-of-the-art automation, a new automated robotic sample loading system, and a CMOS camera purposely designed for SPA. This unique combination enables effortless and efficient sample screening. In addition, the Tundra Cryo-TEM is also capable of resolving the structure of proteins and macromolecules in high resolution. It pioneers high-resolution SPA at low accelerating voltage (keV).
-->Academic and industrial multi-user laboratories that need a high-resolution SEM often select the Apreo 2 SEM because of its ability to image and analyze a wide array of samples. This Tech Spotlight presentation will illustrate Apreo's versatility and the features that make it possible to obtain high quality images on any material—even those typically considered the most challenging.
Learn how our DualBeam system with a fully integrated femtosecond laser allows for millimeter-scale subsurface and 3D analysis at nanometer resolution. This enables new workflows such as fast characterization of air-sensitive samples and multi-scale correlative microscopy with deep subsurface sample extraction. It produces very high-quality cut face suitable for SEM imaging or even surface-sensitive EBSD mapping directly after the laser processing.
Most materials used in clean energy applications are sensitive to air and water, especially battery materials such as Li-metal, solid-state electrolyte, Li-salts or electrode assemblies. An optimum sample preparation and sample transfer process between various instruments that does not compromise sample integrity is crucial for materials characterization. The Thermo Scientific IGST (inert gas sample transfer) Workflow uses tools like the Thermo Scientific CleanConnect sample transfer system to allow users to focus on their battery or clean energy materials research rather than looking for sample contamination due to improper sample handling. This short talk will discuss CleanConnect and is features/applications and introduce the IGST Workflow.
The unique combination of the Thermo Scientific Krios Cryo-TEM, E-CFEG, Selectris Energy Filter, and the latest-generation Falcon 4i Direct Electron Detector has enabled new scientific insights through enhanced productivity and performance of data generation. In this presentation, we will dissect the individual contributions of these four key components (aka the "Power of Four") and talk about the latest software developments that facilitate fast and easy data acquisition. In addition, we will show some of the groundbreaking results that have revolutionized our scientific insights in some of the most challenging research topics.
Preparation of a thin, Ga-free S/TEM sample using focused ion beam (FIB) milling is still considered a challenging task that requires a high level of expertise. The Thermo Scientific Helios 5 Hydra DualBeam, in combination with Thermo Scientific AutoTEM 5 Software, significantly simplifies the workflow and enables automated processing of high-quality, Ga-free lamella for a broad range of materials. The Helios 5 Hydra DualBeam's unique capability to tailor the ion species to specific materials for best results, along with its low-energy polishing (down to 500 eV), makes it best-in-class for site-specific TEM sample prep.
Combining the Thermo Scientific Helios Hydra Plasma FIB DualBeam with a cryo stage and Thermo Scientific Auto Slice & View 5 Software enables new possibilities for imaging frozen hydrated biological samples. The multi-ion species plasma FIB column enables both large-volume milling of cells and tissues and precise, artifact-free milling for lamella. The Thermo Scientific Elstar SEM Column is optimized for biological samples and reduces typical charging-related artifacts. Here, we will show large volumes from high-pressure frozen mice brain tissue and cellular samples showing high-resolution details of structural features at high contrast.
Thermo Scientific ChemiSEM Technology centralizes EDS into the heart of the SEM workflow, enabling real time navigation by chemistry. This Tech Spotlight presents an overview of the technology used within ChemiSEM, and how they are used to solve complex problems across materials science. We will discuss: • How ChemiSEM Technology provides the shortest time to data with an 'always on' workflow • The benefits of live chemical imaging with peak deconvolution for separating challenging element overlaps • How ChemiSEM Technology can be combined with phase and particle analysis for a complete understanding of your specimen
There is a growing need for sub-nanometer resolution to aid metrology of layers of 1 nm thickness. This session discusses solutions that can provide 3D information from thin samples at atomic resolution.
The new Spin Mill functionality of Thermo Scientific Auto Slice & View 5 Software on the Thermo Scientific Helios 5 Hydra DualBeam opens access to new dimensions in 3D characterization. Areas of up to 1 mm in diameter can be exposed and analyzed in high resolution in 3D, including EDS quantification. Availability of Ar as an ion source allows you to obtain curtain-free cuts of the highest quality. Automated low-kV polishing of large areas is another application of the Spin Mill that allows you to prepare surfaces of extremely high quality for EBSD analyses, for example.
Learn how our DualBeam system with a fully integrated femtosecond laser allows for millimeter-scale subsurface and 3D analysis at nanometer resolution. This enables new workflows such as fast characterization of air-sensitive samples and multi-scale correlative microscopy with deep subsurface sample extraction. It produces very high-quality cut face suitable for SEM imaging or even surface-sensitive EBSD mapping directly after the laser processing.
Support for your electron microscope goes far beyond just break/fix maintenance. By integrating digital and artificial intelligence elements, our services can help you improve the speed and reliability of your workflow and optimize the performance of your system. Learn how Connected Care Services can: • Track progress toward your goals • Validate your workflow using a biological sample • Provide insights to pinpoint training needs • Analyze system performance and utilization over time • Minimize unplanned downtime with predictive service monitoring
Failure analysis of semiconductor devices is often complex, requiring multiple standalone instruments to achieve a holistic characterization of the failure. For example, site-specific TEM sample preparation for site-specific failure analysis requires a combination of unique equipment for failure localization, sample preparation and data acquisition. Organization of multiple instruments creates challenges with equipment footprint and multiple operator coordination for material and data handling. In this session, we will discuss the integration of key workflow pieces, such as in a single FIB-SEM platform to eleminate these challenges while improving effectivity and operation practicality.
Meet our new cryo-transmission electron microscope, the Thermo Scientific Tundra Cryo-TEM, dedicated to single particle analysis (SPA). It features state-of-the-art automation, a new automated robotic sample loading system, and a CMOS camera purposely designed for SPA. This unique combination enables effortless and efficient sample screening. In addition, the Tundra Cryo-TEM is also capable of resolving the structure of proteins and macromolecules in high resolution. It pioneers high-resolution SPA at low accelerating voltage (keV).
Single particle analysis (SPA) is an increasingly popular technique for visualizing the structures of proteins and protein complexes. Recent improvements in instruments and automation enhance the efficiency with which high-quality data can be obtained. However, acquiring high-quality SPA data still largely depends on operator expertise. Here, we show how Thermo Scientific Smart EPU Software for our cryo-TEM systems allows for further automation and ease of use, freeing you from tedious, repetitive tasks and opening cryo-EM to a wider audience.
The unique combination of the Thermo Scientific Krios Cryo-TEM, E-CFEG, Selectris Energy Filter, and the latest-generation Falcon 4i Direct Electron Detector has enabled new scientific insights through enhanced productivity and performance of data generation. In this presentation, we will dissect the individual contributions of these four key components (aka the "Power of Four") and talk about the latest software developments that facilitate fast and easy data acquisition. In addition, we will show some of the groundbreaking results that have revolutionized our scientific insights in some of the most challenging research topics.
Understanding both the chemistry and structure of surfaces can be key to understanding how they behave in an application. Join us for this presentation to see how data from XPS instruments, such as the Thermo Scientific Nexsa G2 Surface Analysis System, can be combined with imaging from SEMs by using Thermo Scientific Maps Software. We'll show just how easy it is to correlate the data from the two instruments using our dedicated Thermo Scientific CISA Workflow.
Combining the Thermo Scientific Helios Hydra Plasma FIB DualBeam with a cryo stage and Thermo Scientific Auto Slice & View 5 Software enables new possibilities for imaging frozen hydrated biological samples. The multi-ion species plasma FIB column enables both large-volume milling of cells and tissues and precise, artifact-free milling for lamella. The Thermo Scientific Elstar SEM Column is optimized for biological samples and reduces typical charging-related artifacts. Here, we will show large volumes from high-pressure frozen mice brain tissue and cellular samples showing high-resolution details of structural features at high contrast.
Most materials used in clean energy applications are sensitive to air and water, especially battery materials such as Li-metal, solid-state electrolyte, Li-salts or electrode assemblies. An optimum sample preparation and sample transfer process between various instruments that does not compromise sample integrity is crucial for materials characterization. The Thermo Scientific IGST (inert gas sample transfer) Workflow uses tools like the Thermo Scientific CleanConnect sample transfer system to allow users to focus on their battery or clean energy materials research rather than looking for sample contamination due to improper sample handling. This short talk will discuss CleanConnect and is features/applications and introduce the IGST Workflow.
With Thermo Scientific Avizo 3D Pro Software, you can segment, quantify, and obtain statistical data from SEM images or study large, representative 3D structures and pore space characterization from 3D scans. Degradation and cycling studies of pre and post can be studied with similar image processing, deep learning, and automation recipes to remove biases and obtain quantitative information. In this presentation we will show a typical use-case, its recipe for segmentation, and how you can extract quantitative data out of high-resolution EM images.
Academic and industrial multi-user laboratories that need a high-resolution SEM often select the Apreo 2 SEM because of its ability to image and analyze a wide array of samples. This Tech Spotlight presentation will illustrate Apreo's versatility and the features that make it possible to obtain high quality images on any material—even those typically considered the most challenging.
Preparation of a thin, Ga-free S/TEM sample using focused ion beam (FIB) milling is still considered a challenging task that requires a high level of expertise. The Thermo Scientific Helios 5 Hydra DualBeam, in combination with Thermo Scientific AutoTEM 5 Software, significantly simplifies the workflow and enables automated processing of high-quality, Ga-free lamella for a broad range of materials. The Helios 5 Hydra DualBeam's unique capability to tailor the ion species to specific materials for best results, along with its low-energy polishing (down to 500 eV), makes it best-in-class for site-specific TEM sample prep.
Cross-linking mass spectrometry (XL-MS) approaches offer distinct advantages due to speed, accuracy, sensitivity, and versatility, especially for the study of heterogeneous and dynamic protein complexes. In this presentation, we will introduce the development of a complete, easy-to-use XL-MS workflow going from sample prep to data analysis and incorporating results into an integrative modeling platform via Chimera tools. In addition, examples of the use of XL-MS in characterizing protein-nucleic acid interactions and structural dynamics of protein complexes for both SPA and cryo-tomography applications will be presented.
With Thermo Scientific Amira 3D Pro Software, you can create rapid ground-truth with semi-automated, user-in-the-loop-based segmentation and other tools to help train deep learning models, which can then be applied in the form of recipes to automate and perform large-volume segmentation and analysis. In this presentation, we will show a typical use case and demonstrate how to use traditional image processing and manual and semi-automatic segmentation within the same software tool to extract quantitative data post-acquisition of EM images.
Researchers and engineers across industry and academia rely on the unparalleled spatial resolution of S/TEM imaging for key structural and compositional data to optimize their material’s performance, increasing the demand for more flexible, reliable instrumentation. The Thermo Scientific Talos TEM allows you to spend less time on setting up the microscope with automated daily alignments, easing the learning curve for novice operators and improving time-to-data for the experienced operator. A new ultra-high-brightness cold FEG source reduces the effort to acquire great images and analytics by improving the signal to noise ratio especially nanoparticles or atomic structures. Our Automated Particle Workflow (APW) frees up your TEM for other research and increase the statistical relevance of your nanoparticle analysis by fully automating the workflow from acquisition to particle analysis. Join our presentation and learn more about our workhorse Talos TEM!
The new Embedded CryoSPARC Live, now fully integrated with the Smart EPU ecosystem, can analyze data for single particle analysis (SPA) in real time as it is collected from a Thermo Scientific cryo-transmission electron microscope (cryo-TEM), making it possible to quickly evaluate image quality and decide which samples to carry forward for analysis. Experts from Structura Biotechnolgy and Thermo Fisher Scientific will show how this solution enables users of all experience levels to obtain high-quality data, while reducing the time it takes to transform raw data into high-quality 3D protein structures from days to hours.
We will show some of the highest resolution structures of proteins obtained either from purified samples or directly from cellular lamellas using a Thermo Scientific Krios G4 Cryo-TEM equipped with the Selectris X Imaging Filter and Falcon 4i Direct Electron Detector. In addition, we will show a high-throughput, semi-automated workflow that enables preparation of cellular lamella and data collection with Thermo Scientific TEM Tomography 5 Software, as well as our new on-the-fly tilt series alignment and reconstruction pipeline with Thermo Scientific Tomo Live Software.
Thermo Scientific ChemiSEM Technology centralizes EDS into the heart of the SEM workflow, enabling real time navigation by chemistry. This Tech Spotlight presents an overview of the technology used within ChemiSEM, and how they are used to solve complex problems across materials science. We will discuss: • How ChemiSEM Technology provides the shortest time to data with an 'always on' workflow • The benefits of live chemical imaging with peak deconvolution for separating challenging element overlaps • How ChemiSEM Technology can be combined with phase and particle analysis for a complete understanding of your specimen
Meet our new cryo-transmission electron microscope, the Thermo Scientific Tundra Cryo-TEM, dedicated to single particle analysis (SPA). It features state-of-the-art automation, a new automated robotic sample loading system, and a CMOS camera purposely designed for SPA. This unique combination enables effortless and efficient sample screening. In addition, the Tundra Cryo-TEM is also capable of resolving the structure of proteins and macromolecules in high resolution. It pioneers high-resolution SPA at low accelerating voltage (keV).
The new Spin Mill functionality of Thermo Scientific Auto Slice & View 5 Software on the Thermo Scientific Helios 5 Hydra DualBeam opens access to new dimensions in 3D characterization. Areas of up to 1 mm in diameter can be exposed and analyzed in high resolution in 3D, including EDS quantification. Availability of Ar as an ion source allows you to obtain curtain-free cuts of the highest quality. Automated low-kV polishing of large areas is another application of the Spin Mill that allows you to prepare surfaces of extremely high quality for EBSD analyses, for example.
Cross-linking mass spectrometry (XL-MS) approaches offer distinct advantages due to speed, accuracy, sensitivity, and versatility, especially for the study of heterogeneous and dynamic protein complexes. In this presentation, we will introduce the development of a complete, easy-to-use XL-MS workflow going from sample prep to data analysis and incorporating results into an integrative modeling platform via Chimera tools. In addition, examples of the use of XL-MS in characterizing protein-nucleic acid interactions and structural dynamics of protein complexes for both SPA and cryo-tomography applications will be presented.
We will present a further step in cryo-electron microscope technology development. Analogous to the development of the automated Thermo Scientific Krios Cryo-TEM approximately 15 years ago, we will discuss a next-generation cryo-FIB system. The new instrument is dedicated to production of cryo-lamellae for the cryo-ET workflow. It features a state-of-the-art plasma ion source, an automated sample loading system, integrated fluorescence microscopy, and the lowest contamination rates, allowing preparation of cryo-lamellae at a higher level of productivity than ever before, thus producing the best possible samples for tomography.
Register for personalized demos
We are excited to be able to bring the following products to M&M to perform live demonstrations. If you are interested in seeing something in particular in action, please register in advance for a demo slot. Whether you are in Life Sciences, Materials Science, Semiconductors or another field, our live demos will feature something for every type of researcher.
Come see the 200 kV cryoTEM configuration that is shattering performance barriers. The new Thermo Scientific Smart EPU Software ecosystem makes it easy for you to optimize data collection and monitor data quality, while the Thermo Scientific Selectris Imaging Filter gains remarkable resolution improvements.
The Thermo Scientific Axia ChemiSEM is a newly designed SEM focused on productivity. Its advanced automated functions make it possible for many users to capture a high-resolution image immediately. Thanks to the fully integrated EDS, live quantification is switched on with just one click. SEM imaging combined with reliable, always-on, and fast EDS data—in just over two minutes.
The Thermo Scientific Phenom Pharos G2 Desktop FEG-SEM brings field emission SEM to your tabletop. For labs that do not consider FEG-SEM a realistic option, high-performance imaging and analysis is now accessible thanks to its attractive form factor and short training time. With blazing fast sample exchange, the Phenom Pharos G2 Desktop FEG-SEM is highly productive. Unlike other SEMs, which are often fully booked, it performs imaging and analysis jobs so quickly that it serves well as a walk-up tool. This session will demonstrate the speed, ease of operation, and advanced performance of this desktop FEG-SEM on a range of specimen types.
Register for a demo and discover how the Thermo Scientific Spectra Ultra S/TEM helps you retrieve chemical information quickly, accurately and with the highest efficiency thanks to the Ultra-X EDX solution. Combined with advanced imaging techniques, the Spectra Ultra is the tool for complete characterization of materials, especially those prone to beam damage.
You’ve heard about it, and now is the time to see it in action. The Thermo Scientific Tundra Cryo-TEM now makes it easy for operators of any level to get started in cryo-EM. The semi-automated loader facilitates sample loading, and a series of smart automation features simplifies the data collection process from grid review to single particle collection and data management.
Discover how to achieve high-quality, large-scale cross-sectioning, large-volume 3D characterization, and Ga-free S/TEM sample preparation using the latest multi-ion species plasma FIB technology. The innovative Thermo Scientific Helios 5 Hydra DualBeam, in combination with advanced software, brings the highest level of automation and allows users with any experience level to obtain expert-level results, even for most challenging samples in materials science. Join this demonstration to learn how the Helios 5 Hydra DualBeam can help address your research challenges.
Join our booth live demo to learn more about our workhorse Thermo Scientific Talos TEM! In this demo, we will show you:
Wherever imaging data sets need to be processed and managed, Thermo Scientific Software solutions offer abundant state-of-the-art image processing, exploration and analysis features. Our adaptable software packages dedicated to life sciences and materials science make complex visualization, analysis and data management of image data faster and easier for a wide range of applications. Come see how latest versions of Amira and Avizo data analysis software offer most comprehensive and versatile solutions for all your imaging data analysis workflows.
Multi-scale and multi-modal correlative microscopy methods, which involve the coordinated characterization of materials across a range of length scales using various apparatus, allow solving a broad range of scientific problems previously unreachable by the typical experimental operando.
DownloadWe demonstrate the differences in 3D and 2D convolutional U-Net networks by showing results of training models on a publicly available dataset. The dataset consists of training and testing sub volumes all with separate manually marked mitochondrial structures as part of a neuroscience study of a CA1 hippocampus region of a mouse brain. Our initial analysis of 3D vs 2D U-Net shows that 3D U-Net can capture the morphological structures better than a 2D U-Net We have presented this evidence in the form of segmentation metrics compared to unseen during training, a testing image stack The improvements in precision precision are about 39 and a higher F1 and Accuracy score of 24% and 4% respectively.
DownloadIn electron microcopy, automation and advanced user-customized software control have great potential to provide new insights in material science. Scripting allows the user to try out unique techniques and experimental workflows to explore new scientific ideas. This opens a new field of experiments and solutions not easily accessible with commercial software. For this reason,Thermo Fisher Scientific has made the established Autoscript software (used already on dualbeamsystems) available for transmission electron microscope (TEM) systems.
DownloadTrained Neural Networks allow to automaticallycontrol the acquisitionandanalysis process of multimodal STEM techniques. This provides the benefit of faster time to data, lower dose for minimizing damage on weakmultimodal signals like spectroscopyand highly reproducible metrology on the obtained data.
DownloadSPA as well as Cryo-ET have seen massive investment leading to a multitude of technological advances leading to significantly improved ease-of-use and data collection throughput. While the workhorse 200kV Cryo-TEMs have been playing an important role in SPA screening for the most promising samples and, on occasion, solving some SPA highresolution structures we believe that 200 kV systems with energy filters has the potential to bring routine high-throughput, high–resolution Cryo-EM to the masses.
DownloadFIB-SEM nanotomography has been established as a key methodological approach for the 3D analysis of biological ultrastructure at isotropic voxel resolutions. The technique is based on the acquisition of thousands of high-resolution SEM images, but its applicability can be limited by the individual image acquisition time, which may take up to a few minutes per frame. We have developed two different methods that leverage AI algorithms, to reduce the acquisition time of 3D datasets by two alternate approaches.
DownloadHistorically High Angle Annular Dark Field or HAADF has been the dominant contrast mechanism in STEM. However, in the last decade Bright Field (BF) imaging in STEM has enjoyed a renaissance because contrary to Dark Field (DF) techniques they allow for many more transmitted electrons to participate in the imaging process. As such the BF techniques perform much better than the DF ones in low-dose conditions. Unfortunately, none of the BF techniques are linear, their Contrast Transfer Functions (CTF) are object dependent and retrieving the object from the image is all but trivial because BF contrast changes with sample thickness and defocus.
DownloadModern EDS (energy-dispersive spectroscopy) X-ray microanalysis systems enable the acquisition of EDS hyperspectral datasets over entire samples. From these datasets, various maps can be extracted including qualitative and quantitative elemental distribution maps and phase maps. Here, I explore current possibilities and challenges for large area, standards-based quantitative EDS mapping.
DownloadComing Soon
In recent years ultra short pulse lasers (USP Lasers) have been integrated with Focused Ion Beam (FIB) and Plasma FIB instruments. The combined technology enables rapid millimeter size cross sectional milling, imaging (2D) and consecutive slicing (3D). The instrument used for the experiments described here is a ThermoFisher Laser PFIB. With this instrument one would work at a single coincident point for the Scanning Electron Microscope (SEM), the PFIB and the USP Laser. Intermittent imaging with only a few seconds between laser milling and SEM imaging allows for fast and easy parameter optimization. This systems supports 2 wavelengths, namely 515nm and 1030nm.
DownloadComing Soon
In-booth Session Presentations
Below are the video presentations presented by Thermo Fisher Scientific at M&M 2022.
We will show some of the highest resolution structures of proteins obtained either from purified samples or directly from cellular lamellas using a Thermo Scientific Krios G4 Cryo-TEM equipped with the Selectris X Imaging Filter and Falcon 4i Direct Electron Detector. In addition, we will show a high-throughput, semi-automated workflow that enables preparation of cellular lamella and data collection with Thermo Scientific TEM Tomography 5 Software, as well as our new on-the-fly tilt series alignment and reconstruction pipeline with Thermo Scientific Tomo Live Software.
Resources unavailableUnderstanding both the chemistry and structure of surfaces can be key to understanding how they behave in an application. Join us for this presentation to see how data from XPS instruments, such as the Thermo Scientific Nexsa G2 Surface Analysis System, can be combined with imaging from SEMs by using Thermo Scientific Maps Software. We'll show just how easy it is to correlate the data from the two instruments using our dedicated Thermo Scientific CISA Workflow.
Watch DownloadMeet our new cryo-transmission electron microscope, the Thermo Scientific Tundra Cryo-TEM, dedicated to single particle analysis (SPA). It features state-of-the-art automation, a new automated robotic sample loading system, and a CMOS camera purposely designed for SPA. This unique combination enables effortless and efficient sample screening. In addition, the Tundra Cryo-TEM is also capable of resolving the structure of proteins and macromolecules in high resolution. It pioneers high-resolution SPA at low accelerating voltage (keV).
WatchAcademic and industrial multi-user laboratories that need a high-resolution SEM often select the Apreo 2 SEM because of its ability to image and analyze a wide array of samples. This Tech Spotlight presentation will illustrate Apreo's versatility and the features that make it possible to obtain high quality images on any material—even those typically considered the most challenging.
Watch DownloadLearn how our DualBeam system with a fully integrated femtosecond laser allows for millimeter-scale subsurface and 3D analysis at nanometer resolution. This enables new workflows such as fast characterization of air-sensitive samples and multi-scale correlative microscopy with deep subsurface sample extraction. It produces very high-quality cut face suitable for SEM imaging or even surface-sensitive EBSD mapping directly after the laser processing.
Learn moreMost materials used in clean energy applications are sensitive to air and water, especially battery materials such as Li-metal, solid-state electrolyte, Li-salts or electrode assemblies. An optimum sample preparation and sample transfer process between various instruments that does not compromise sample integrity is crucial for materials characterization. The Thermo Scientific IGST (inert gas sample transfer) Workflow uses tools like the Thermo Scientific CleanConnect sample transfer system to allow users to focus on their battery or clean energy materials research rather than looking for sample contamination due to improper sample handling. This short talk will discuss CleanConnect and is features/applications and introduce the IGST Workflow.
Watch DownloadThe unique combination of the Thermo Scientific Krios Cryo-TEM, E-CFEG, Selectris Energy Filter, and the latest-generation Falcon 4i Direct Electron Detector has enabled new scientific insights through enhanced productivity and performance of data generation. In this presentation, we will dissect the individual contributions of these four key components (aka the "Power of Four") and talk about the latest software developments that facilitate fast and easy data acquisition. In addition, we will show some of the groundbreaking results that have revolutionized our scientific insights in some of the most challenging research topics.
WatchPreparation of a thin, Ga-free S/TEM sample using focused ion beam (FIB) milling is still considered a challenging task that requires a high level of expertise. The Thermo Scientific Helios 5 Hydra DualBeam, in combination with Thermo Scientific AutoTEM 5 Software, significantly simplifies the workflow and enables automated processing of high-quality, Ga-free lamella for a broad range of materials. The Helios 5 Hydra DualBeam's unique capability to tailor the ion species to specific materials for best results, along with its low-energy polishing (down to 500 eV), makes it best-in-class for site-specific TEM sample prep.
Learn moreCombining the Thermo Scientific Helios Hydra Plasma FIB DualBeam with a cryo stage and Thermo Scientific Auto Slice & View 5 Software enables new possibilities for imaging frozen hydrated biological samples. The multi-ion species plasma FIB column enables both large-volume milling of cells and tissues and precise, artifact-free milling for lamella. The Thermo Scientific Elstar SEM Column is optimized for biological samples and reduces typical charging-related artifacts. Here, we will show large volumes from high-pressure frozen mice brain tissue and cellular samples showing high-resolution details of structural features at high contrast.
WatchThermo Scientific ChemiSEM Technology centralizes EDS into the heart of the SEM workflow, enabling real time navigation by chemistry. This Tech Spotlight presents an overview of the technology used within ChemiSEM, and how they are used to solve complex problems across materials science. We will discuss:
There is a growing need for sub-nanometer resolution to aid metrology of layers of 1 nm thickness. This session discusses solutions that can provide 3D information from thin samples at atomic resolution.
Learn moreSupport for your electron microscope goes far beyond just break/fix maintenance. By integrating digital and artificial intelligence elements, our services can help you improve the speed and reliability of your workflow and optimize the performance of your system. Learn how Connected Care Services can:
Failure analysis of semiconductor devices is often complex, requiring multiple standalone instruments to achieve a holistic characterization of the failure. For example, site-specific TEM sample preparation for site-specific failure analysis requires a combination of unique equipment for failure localization, sample preparation and data acquisition. Organization of multiple instruments creates challenges with equipment footprint and multiple operator coordination for material and data handling. In this session, we will discuss the integration of key workflow pieces, such as in a single FIB-SEM platform to eleminate these challenges while improving effectivity and operation practicality.
Watch DownloadSingle particle analysis (SPA) is an increasingly popular technique for visualizing the structures of proteins and protein complexes. Recent improvements in instruments and automation enhance the efficiency with which high-quality data can be obtained. However, acquiring high-quality SPA data still largely depends on operator expertise. Here, we show how Thermo Scientific Smart EPU Software for our cryo-TEM systems allows for further automation and ease of use, freeing you from tedious, repetitive tasks and opening cryo-EM to a wider audience.
WatchWith Thermo Scientific Avizo 3D Pro Software, you can segment, quantify, and obtain statistical data from SEM images or study large, representative 3D structures and pore space characterization from 3D scans. Degradation and cycling studies of pre and post can be studied with similar image processing, deep learning, and automation recipes to remove biases and obtain quantitative information. In this presentation we will show a typical use-case, its recipe for segmentation, and how you can extract quantitative data out of high-resolution EM images.
WatchCross-linking mass spectrometry (XL-MS) approaches offer distinct advantages due to speed, accuracy, sensitivity, and versatility, especially for the study of heterogeneous and dynamic protein complexes. In this presentation, we will introduce the development of a complete, easy-to-use XL-MS workflow going from sample prep to data analysis and incorporating results into an integrative modeling platform via Chimera tools. In addition, examples of the use of XL-MS in characterizing protein-nucleic acid interactions and structural dynamics of protein complexes for both SPA and cryo-tomography applications will be presented.
Watch DownloadWith Thermo Scientific Amira 3D Pro Software, you can create rapid ground-truth with semi-automated, user-in-the-loop-based segmentation and other tools to help train deep learning models, which can then be applied in the form of recipes to automate and perform large-volume segmentation and analysis. In this presentation, we will show a typical use case and demonstrate how to use traditional image processing and manual and semi-automatic segmentation within the same software tool to extract quantitative data post-acquisition of EM images.
Watch DownloadResearchers and engineers across industry and academia rely on the unparalleled spatial resolution of S/TEM imaging for key structural and compositional data to optimize their material’s performance, increasing the demand for more flexible, reliable instrumentation. The Thermo Scientific Talos TEM allows you to spend less time on setting up the microscope with automated daily alignments, easing the learning curve for novice operators and improving time-to-data for the experienced operator. A new ultra-high-brightness cold FEG source reduces the effort to acquire great images and analytics by improving the signal to noise ratio especially nanoparticles or atomic structures. Our Automated Particle Workflow (APW) frees up your TEM for other research and increase the statistical relevance of your nanoparticle analysis by fully automating the workflow from acquisition to particle analysis. Join our presentation and learn more about our workhorse Talos TEM!
WatchCustomer appreciation reception
Thank you to all who joined us as we celebrated you—our customers with and evening of food, refreshments, and socializing!
Eastlund Hotel
Met Tavern rooftop restaurant
1021 NE Grand Ave
Portland, OR 97232
Women in Microscopy breakfast
Karren More received her PhD in 1992 from the Department of Materials Science and Engineering at North Carolina State University, Raleigh, NC, under the direction of Professor Robert F. Davis (now at Carnegie Mellon University). She joined Oak Ridge National Laboratory (ORNL) as a full-time research staff member in 1988 and, since joining ORNL, has focused her research on high-resolution analytical electron microscopy of a variety of material systems, including structural ceramics, ceramic matrix composites, nanoparticle catalysts, and polymer electrolyte fuel cells. Karren's successful research career at ORNL has resulted in nearly 300 publications, an h-index of 65, and more than 20,000 citations; in 2021, she was named a Highly Cited Researcher for 2020 by Clarivate Analytics. Karren is a Fellow of the American Ceramic Society (Class of 2007) and the Microscopy Society of America (Class of 2019) and was recently inducted into the NCSU Department of Materials Science & Engineering Hall of Fame (2022). Karren served as the Group Leader of the Electron & Atom Probe Microscopy Group in ORNL’s Center for Nanophase Materials Sciences (CNMS) for approximately 10 years. In 2019, Karren was named Director of the Center for Nanophase Materials Sciences at ORNL.
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Microscopy & Microanalysis 2022
Oregon Convention Center
777 NE Martin Luther King Jr Blvd.
Portland, OR 97232
Mon., Aug. 1 – Thu., Aug. 4
Yes, I would like to speak with an expert to learn how Thermo Scientific products can help maximize productivity in my laboratory.