MC 2019
Our in-booth theater presentations include dynamic solution-focused presentations on Thermo Fisher’s innovative technologies and workflow solutions. Participants will be able to engage with and learn directly from technology and application experts.
To sign up: Select each presentation you would like to attend and click Register.
While virtually all modern imagery is in color, conventional scanning electron microscopy (SEM) is still a greyscale technique. This has led many users of SEM to add color with artificial methods, even when these colors do not carry a scientific meaning. A technique such as energy-dispersive x-ray spectroscopy (EDS) is able to provide SEM images with meaningful color, but it usually requires a separate data processing chain and separate software which makes it difficult to use and slow. Here we present live color SEM imaging with colors based on elemental composition. Working with live color images is the most intuitive form of elemental analysis, it provides direct insight into compositional differences that might otherwise go unnoticed, and it provides the shortest time to analytical result. Employing novel data processing algorithms, ColorSEM is a completely new way of processing EDS information in a system that has no barriers to new users, while providing images with a color that is actually meaningful.
RegisterThermo Fisher Scientific introduces the new Thermo Scientific™ Spectra scanning/transmission electron microscope designed to accelerate breakthrough discoveries by delivering advanced atomic-scale imaging and analysis, all in one tool. Spectra S/TEM combines the highest commercially available image resolution specification with the highest sensitivity, and allows users of all experience levels to generate best-in-class results. New features include a higher-brightness electron source (bright cold field emission gun (X-CFEG)) for higher contrast imaging. The revolutionary detection capabilities allow scientists and engineers to acquire previously unobtainable atomic-level data for a wider range of applications, including extremely beam-sensitive materials such as metal organic frameworks, zeolites, and polymers. It also meets the growing demand for high-volumes of atomic-level chemical analysis using several modalities such as EDX (Energy Dispersive X-Ray) or EELS (Electron Energy Loss Spectroscopy).
RegisterWindowless and racetrack EDS detectors present a step change in analytical performance across a range of application areas, providing the analyst with such benefits as an increased efficiency of collection, improved light element sensitivity and enhanced detector throughput. Such detectors also necessitate advanced algorithmic approaches to enable accurate quantification even in the presence of significant peak overlaps, such as modified Phi-rho-Z matrix correction routines, advanced peak filtering and intelligent statistical mapping. This presentation explores how Thermo Scientific Pathfinder can be used to get the most from the latest EDS technical developments for the ultimate in analytical performance.
RegisterIn this session we will present the latest advances of the Falcon series of direct electron detectors, delivering improved image quality and acquisition speed. Combined with the ease-of-use and advanced automation of the acquisition software EPU 2, it is the ideal solution for the single particle analysis workflow (SPA). To improve the user experience further, enhanced server hardware and new software capabilities facilitate SPA data management and remote, one-the-fly quality monitoring. Join this session to learn how combining the Falcon and EPU with data management and on-the-fly monitoring of data quality improves the efficiency and productivity of SPA data acquisition so that users spend less time on data collection while being confident of getting the best data
RegisterThe Aquilos cryo-FIB microscope brings the structural cell biologist a preparation tool which allows preparing optimal samples for cryo-electron tomography and opening windows into the native cellular environment. The continuous development of this platform brings more ease of use, enables better sample throughput and opens new application areas. Here, we will present the latest advances that make cryo-FIB technology key for the exploration of biomolecules in the context of cellular structures.
RegisterNew Thermo Scientific™ Avizo2D Software is designed to create AI-powered automation recipes for use online during acquisition. It features an end-to-end deep learning (DL) pipeline for creating ground-truth data and training models using state-of-the-art architectures, such as Unet, from a highly configurable tool. Trained models can then be used as SNR engines or automated segmentation methods for use cases that cannot be solved with traditional approaches. Once designed, Avizo2D AI-powered recipes can be used during acquisition, enabling automated imaging and analysis workflows using the most advanced image processing solutions.
RegisterDirect quantitative revelation of three-dimension morphology is of great importance in understanding the inner microstructure and properties of polymer materials. Visualizing the entire membrane at high resolution is key to understand how regions with different microstructures affect filtration behaviour and ultimately predict filtration performance. By integrating an in situ ultramicrotomy and electron microscopy, we provide a fully automated workflow which allows the acquisition of high-resolution spatial SEM micrographs during serial sectioning using the microtome. The method is also known as serial block face SEM (SBFSEM). With the subsequent 3D reconstruction of the micrograph stacks, we can recover the comprehensive volume information of the polymer materials. Thermo Scientific™ Apreo VolumeScope combines the field of view of a microtome with the resolution of a powerful SEM, allowing us to conduct fast, reliable and high resolution, high contrast 3D imaging of soft materials.
RegisterStructural biologists have made great progress in discovering the structures of individual proteins and protein complexes. Much of the recent progress has resulted from advances in cryo-TEM, especially single-particle analysis, which has made the elucidation of near-atomic-scale structures for small to large proteins and protein complexes almost routine. The ultimate goal is to derive the structures of the molecular machines that constitute all living systems in their natural, functional contexts, where their interactions with other components can be observed. Studying the molecular machinery of cells from atomic detail to the cellular context and beyond is a great challenge for cell biology. This workshop will highlight the latest developments in cryo-electron microscopy. Special attention is given to our advances on productivity enhancements in the context of the Single Particle Analysis (SPA) workflow. Secondly, we will introduce the suite of instruments that enable Thermo Fisher Scientific's cryo-electron tomography workflow which allow to peer inside cells and see proteins in situ – in their unperturbed functional environments – at nanometer resolution and in 3D
RegisterPreparation of a thin S/TEM sample using FIB (focused ion beam) is still considered a challenging task, requiring an operator to possess a high level of expertise and extensive training. The newest generation Thermo Scientific™ Helios™ 5 DualBeam, in combination with Thermo Scientific™ AutoTEM™ 5 Software and dedicated Accelerate & Advance service support offerings, enables a fully automated process of highest quality lamella preparation—from chunking to in situ lift-out, thinning and final low-kV polishing. It is designed to be reliably applied to a wide range of difficult materials. In full automation mode, it can prepare thinned lamella, unattended, as quickly as 45 minutes.
RegisterThe Plasma Focused ion Beam (PFIB) combined with a scanning electron microscope (SEM) has been gaining popularity for several years. One of the reasons for this is that the Xe+ PFIB removes material about 38x faster than a Ga+ FIB and allows for ~150 cubic micron 2D cross-sectioning, 3D serial sectioning and Ga+ Free TEM sample preparation. However, when the desired volume grows above 150 cubic micron, the throughput becomes low and alternative removal techniques may be considered to reduce time to result. In order to extend the range of the PFIB, an ultra-short-pulse (USP) laser was recently added as a third beam. Instead of 150 cubic micron materials characterization by PFIB, this range is now extended to well over 1 cubic millimeter. Achievable removal rates of the USP laser surpass 14.000x that of FIB. The PFIB, SEM and USP laser all coincide at the same point in the main vacuum chamber for high throughput in-situ 2D x-sectioning and 3D serial sectioning. A USP laser can cut many materials an order of magnitude faster than just a PFIB. (e.g. metals, glass, ceramics, hard- and soft polymers, graphite, biomaterials etc.) Protective coatings to reduce charge are often not needed due to the absence of specimen charging during material removal. USP laser milled surfaces are clean enough for SEM imaging and often even clean enough for EBSD. An USP laser induces no heat, no microcracks or melting effects. Ultra-fine sub 5nm features can be imaged by SEM after a quick PFIB polish. High precision in-situ feedback and 3D serial sectioning are enabled due to the short laser/SEM switching times. This tutorial describes the technology and the benefits of having an in-situ USP laser on a PFIB DualBeam for materials characterization
RegisterDo you need chemical information on your sample faster? Or working with beam-sensitive materials? The Thermo Scientific Talos F200i scanning/transmission electron microscope (S/TEM) for high-resolution imaging and analysis applications is now available with new symmetrically positioned Dual-X EDS detectors for the highest analytical throughput. Our high-brightness X-FEG gun combined with the high-speed Dual-X EDS detectors, embedded in the intuitive Velox software, enables easy chemical analysis at lower doses on a wider range of samples, including beam-sensitive materials.
RegisterCryo-electron tomography combines the best structural preservation possible with the power of three-dimensional high-resolution imaging. The method allows to peer inside cells and see proteins in situ – in their unperturbed functional environments. We will introduce the suite of instruments that enable Thermo Fisher Scientific's cryo-electron tomography workflow and we will demonstrate how data obtained with this workflow can be visualized using Amira Software. The workshop will feature a live demonstration of Amira Software. Based on a real data set, we will demonstrate how the visualization of cryo-tomography data can be facilitated using semi-automated tools for the detection of filamentous cellular structures and image processing routines for enhancing the segmentation of cellular membranes
RegisterThermo Fisher Scientific introduces the new Thermo Scientific™ Spectra scanning/transmission electron microscope designed to accelerate breakthrough discoveries by delivering advanced atomic-scale imaging and analysis, all in one tool. Spectra S/TEM combines the highest commercially available image resolution specification with the highest sensitivity, and allows users of all experience levels to generate best-in-class results. New features include a higher-brightness electron source (bright cold field emission gun (X-CFEG)) for higher contrast imaging. The revolutionary detection capabilities allow scientists and engineers to acquire previously unobtainable atomic-level data for a wider range of applications, including extremely beam-sensitive materials such as metal organic frameworks, zeolites, and polymers. It also meets the growing demand for high-volumes of atomic-level chemical analysis using several modalities such as EDX (Energy Dispersive X-Ray) or EELS (Electron Energy Loss Spectroscopy).
RegisterIn this session, you will learn how micro electron diffraction (MicroED) allows fast, high-resolution 3D structure determination of small chemical compounds and biological macromolecules. To efficiently collect diffraction datasets of nano-crystals, a cryo-TEM is equipped with a specially designed diffraction camera (Ceta-D) and a MicroED package. The latter combines the necessary hardware components, as well as optimized optical settings and specialized EPU-D Software for automated data collection. Combined with the intrinsic microscope performance, the data collection is fully automated and can be realized in a matter of minutes
RegisterWDS (wavelength-dispersive spectroscopy) X-ray microanalysis provides spectral resolution that is an order of magnitude better than EDS and eliminates many of the peak overlaps that occur in EDS. WDS has also long been regarded as yielding the best quantitative results achievable by X-ray microanalysis. However, the long acquisition times inherent in traditional WDS spectrometer design have resulted in WDS rarely being used as an analytical tool on SEMs. Instead, WDS analysis is only commonly performed on a WDS-specialized microscope, the electron microprobe. Unfortunately, electron microprobes cost 10x more than a WDS spectrometer for an SEM and require an expert operator. The Thermo Scientific MagnaRay WDS spectrometer features innovations in WDS spectrometer technology that make WDS into a microanalytical technique that is practical for SEM users and affordable for microscope facilities.
RegisterUntil recently, only Ga and Xe FIB/SEM instruments were commercially available, drawing significant interest due to their novelty and capabilities. Ga FIB delivers good results for sample preparation and nanoprototyping due to its small spot size and high current density. Xe FIB has a much higher maximum current, which allows high-throughput milling for large volume characterization, as well as Ga-free processing. However, for a number of applications, neither Ga nor Xe could provide the highest quality results. This year, we are introducing the Thermo Scientific™ Helios™ Hydra™ DualBeam, with its new-generation plasma FIB technology supporting multiple ion species as a primary beam. In addition to xenon, the Helios Hydra DualBeam provides three additional ion species: argon, oxygen and nitrogen. This single ion source delivering multiple ion species enables a unique, fast and easy switching capability between different ion species, which will significantly expand the application space and provide advantages to various use cases.
RegisterWe are excited to be able to bring the following products to Microscopy Conference to perform live demonstrations. If you are interested in seeing something in particular in action, please register in advance for a demo slot. Whether you are in Life Sciences, Materials Science, Semiconductors or another field, our live demos will feature something for every type of researcher.
This year, Thermo Fisher combined SEM imaging with live elemental analysis in its new ColorSEM technology. ColorSEM runs on the Prisma E SEM, the most complete SEM for multi-user laboratories that require all-round performance and ease-of-use. With ColorSEM, Prisma E is more complete than ever.
This demonstration is for anyone interested in adding SEM capability to their lab. You will be able to test the robustness, completeness, flexibility and ease of use of Prisma E. And of course, live color imaging will be demonstrated on several sample materials, showing the benefits of ColorSEM in terms of speed, intuitive UI and complete sample information.
Thermo Scientific™ Amira™ Software is an advanced 3D analysis application for exploring and understanding life sciences data. Amira Software enables advanced 3D imaging workflows for specialists in research areas ranging from molecular and cellular biology to neuroscience and bioengineering.
RegisterThermo Scientific™ Avizo™ Software is an advanced 3D analysis application for exploring and understanding materials’ structures and properties, in a wide range of materials science research areas and for many types of materials (metals and alloys, ceramics, composites and polymers).
RegisterThe Thermo Scientific™ Phenom™ XL Desktop SEM pushes the boundaries of compact SEM performance. It features the proven ease-of-use and fast time-to-image of any Phenom system. It is equipped with a chamber that allows analysis of large samples up to 100 mm x 100 mm. A proprietary venting/loading mechanism ensures the fastest vent/load cycle in the world, providing the highest throughput. A newly developed compact motorized stage enables you to scan the full sample area, and yet the Phenom XL Desktop SEM needs little space and no extra facilities.
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Booth 51
Exhibition hall – Der Lichthof
Hours
| Mon., Sep. 2 | 09:00 – 17:30 |
| Tue., Sep. 3 | 09:00 – 17:30 |
| Wed., Sep. 4 | 09:00 – 17:30 |
Microscopy Conference 2019
Technische Universität Berlin
Straße des 17. Juni 135
Berlin, 10623 Germany
Sun., Sep. 1 – Thu., Sep. 5
Yes, I would like to speak with an expert to learn how Thermo Scientific products can help maximize productivity in my laboratory.